Maaß S, Otto A, Albrecht D, Riedel K, Trautwein-Schult A, Becher D. A Proteomic signatures of Clostridium difficile stressed with metronidazole, vancomycin, or fidaxomicin. Cells. 2018.

Stobernack T, du Teil Espina M, Mulder LM, Palma Medina LM, Piebenga DR, Gabarrini G, Zhao X, Janssen KMJ, Hulzebos J, Brouwer E, Sura T, Becher D, van Winkelhoff AJ, Götz F, Otto A, Westra J, van Dijl JM. A Secreted Bacterial Peptidylarginine Deiminase Can Neutralize Human Innate Immune Defenses. MBio. 2018. 9(5): e01704-18. PMID: 30377277.

Götz F, Pjevac P, Markert S, McNichol J, Becher D, Schweder T, Mussmann M, Sievert SM. Transcriptomic and Proteomic Insight into the Mechanism of Cyclooctasulfur- versus Thiosulfate-Oxidation by the Chemolithoautotroph Sulfurimonas denitrificansEnviron Microbiol. 2018. [Epub ahead of print]. PMID: 30362214.

Junker S, Maaß S, Otto A, Hecker M, Becher D. Toward the Quantitative Characterization of Arginine Phosphorylations in Staphylococcus aureus. J Proteome Res. 2018. [Epub ahead of print]. PMID: 30358407.

Trautwein-Schult A, Maaß S, Plate K, Otto A, Becher D. A Metabolic Labeling Strategy for Relative Protein Quantification in Clostridioides difficileFront Microbiol. 2018. eCollection 2018. PMID: 30386308.

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avcı B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H. Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018. [Epub ahead of print]. PMID: 30246424.

Hofmann JD, Otto A, Berges M, Biedendieck R, Michel AM, Becher D, Jahn D, Neumann-Schaal M. Metabolic Reprogramming of Clostridioides difficile During the Stationary Phase With the Induction of Toxin Production. Front Microbiol. 2018. eCollection 2018. PMID: 30186274.

Koch H, Dürwald A, Schweder T, Noriega-Ortega B, Vidal-Melgosa S, Hehemann JH, Dittmar T, Freese HM, Becher D, Simon M, Wietz M. Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2018. [Epub ahead of print]. PMID: 30116038.

Kappelmann L, Krüger K, Hehemann JH, Harder J, Markert S, Unfried F, Becher D, Shapiro N, Schweder T, Amann RI, Teeling H. Polysaccharide utilization loci of North Sea Flavobacteriia as basis for using SusC/D-protein expression for predicting major phytoplankton glycans. ISME J. 2018. [Epub ahead of print]. PMID: 30111868.

Kroniger T, Otto A, Becher D. Proteomic analysis of bacterial (outer) membrane vesicles: progress and clinical potential. Expert Rev. Proteomics. 2018. [Epub ahead of print]. PMID: 30068219.

Unfried F, Becker S, Robb CS, Hehemann JH, Markert S, Heiden SE, Hinzke T, Becher D, Reintjes G, Krüger K, Avcı B, Kappelmann L, Hahnke RL, Fischer T, Harder J, Teeling H, Fuchs B, Barbeyron T, Amann RI, Schweder T. Adaptive mechanisms that provide competitive advantages to marine bacteroidetes during microalgal blooms. The ISME Journal. 2018. [Epub ahead of print]. PMID: 30061707.

Shulman A, Yair Y, Biran D, Sura T, Otto A, Gophna U, Becher D, Hecker M, Ron EZ. The Escherichia coli Type III Secretion System 2 Has a Global Effect on Cell Surface. mBio. 2018. 9(4): e01070-18. PMID: 29970469.

García-Pérez AN, de Jong A, Junker S, Becher D, Chlebowicz MA, Duipmans JC, Jonkman MF, van Dijl JM. From the wound to the bench: exoproteome interplay between wound-colonizing Staphylococcus aureus strains and co-existing bacteria. Virulence. 2018. 9(1):363-378. PMID: 29233035.

Handtke S, Albrecht D, Otto A, Becher D, Hecker M, Voigt B. The proteomic response of B. pumilus cells to glucose starvation. Proteomics. 2018. 18(1).                             PMID: 29193752.

Hentschker C, Dewald C, Otto A, Büttner K, Hecker M, Becher D. Global quantification of phosphoproteins combining metabolic labeling and gel-based proteomics in B. pumilusELECTROPHORESIS. 2018. 39(2):334-343. PMID: 28944503.

Raschdorf O, Bonn F, Zeytuni N, Zarivach R, Becher D, Schüler D. A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle. J Proteomics. 2018. 172:89-99. PMID: 29054541.

Hoyer J, Bartel J, Gómez-Mejia A, Rohde M, Hirschfeld C, Heß N, Sura T, Maaß S, Hammerschmidt S, Becher D. Proteomic response of Streptococcus pneumoniae to iron limitation. Int J Med Microbiol. 2018. [Epub ahead of print]. PMID: 29496408.

Junker S, Maaß S, Otto A, Michalik S, Morgenroth F, Gerth U, Hecker M, Becher D. Spectral library based analysis of arginine phosphorylations in Staphylococcus aureusMol Cell Proteomics. 2018 17:335-348.  PMID: 29183913

Sievers S, Dittmann S, Jordt T, Otto A, Hochgräfe F, Riedel K. Comprehensive redox profiling of the thiol proteome of Clostridium difficileMol Cell Proteomics. 2018. [Epub ahead of print]. PMID: 29496906.

Müller A, Grein F, Otto A, Gries K, Orlov D, Zarubaev V, Girard M, Sher X, Shamova O, Roemer T, François P, Becher D, Schneider T, Sahl HG. Differential daptomycin resistance development in Staphylococcus aureus strains with active and mutated gra regulatory systems. Int J Med Microbiol. 2018. 308(3):335-348.                                  PMID: 29429584.

Pjevac P, Meier DV, Markert S, Hentschker C, Schweder T, Becher D, Gruber-Vodicka HR, Richter M, Bach W, Amann R, Meyerdierks A. Metaproteogenomic Profiling of Microbial Communities Colonizing Actively Venting Hydrothermal Chimneys. Front Microbiol. 2018. 308(3):335-348. PMID: 29696004.

Treffon J, Block D, Moche M, Reiß S, Fuchs S, Engelmann S, Becher D, Langhanki L, Mellmann A, Peters G, Kahl BC. Adaptation of Staphylococcus aureus to the airways of cystic fibrosis patients by the up-regulation of superoxide dismutase M and iron-scavenging proteins. J Infect Dis. 2018. 217(9):1453-1461. PMID: 29325044.


García-Pérez AN, de Jong A, Junker S, Becher D, Chlebowicz MA, Duipmans JC, Jonkman MF, van Dijl JM. From the wound to the bench: exoproteome interplay between wound-colonizing Staphylococcus aureus strains and co-existing bacteria. Virulence. 2017. [Epub ahead of print]. PMID: 29233035.

Junker S, Maaß S, Otto A, Michalik S, Morgenroth F, Gerth U, Hecker M, Becher D. Spectral library based analysis of arginine phosphorylations in Staphylococcus aureusMol Cell Proteomics. 2018. 17:335-348. . PMID: 29183913.

Ünsaldı E, Kurt-Kızıldoğan A, Voigt B, Becher D, Özcengiz G. Proteome-wide alterations in an industrial clavulanic acid producing strain of Streptomyces clavuligerusSynth and Syst Biotechnol. 2017. 2(1):39-48. PMID: 29062960.

Otto A, Maaß S, Bonn F, Büttner K, Becher D. An easy and fast protocol for affinity bead-based protein enrichment and storage of proteome samples. Methods Enzymol. 2017. 585:1-13. PMID: 28109424.

Michalik S, Depke M, Murr A, Gesell Salazar M, Kusebauch U, Sun Z, Meyer TC, Surmann K, Pförtner H, Hildebrandt P, Weiss S, Palma Medina LM, Gutjahr M, Hammer E, Becher D, Pribyl T, Hammerschmidt S, Deutsch EW, Bader SL, Hecker M, Moritz RL, Mäder U, Völker U, Schmidt F. A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions. Sci Rep. 2017. 7(1):9718. PMID: 28887440.

Abendroth U, Adlung N, Otto A, Grüneisen B, Becher D, Bonas U. Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria. BMC Genomics. 2017. 18:625. PMID: 28814272.

Hillion M, Imber M, Pedre B, Bernhardt J, Saleh M, Loi VV, Maaß S, Becher D, Astolfi Rosado L, Adrian L, Weise C, Hell R, Wirtz M, Messens J, Antelmann H. The glyceraldehyde-3-phosphate dehydrogenase GapDH of Corynebacterium diphtheriae is redox-controlled by protein S-mycothiolation under oxidative stress. Sci Rep. 2017. 7:5020. PMID: 28694441.

Arora G, Sajid A, Virmani R, Singhal A, Kumar CMS, Dhasmana N, Khanna T, Maji A, Misra R, Molle V, Becher D, Gerth U, Mande SC, Singh Y. Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis. NPJ Biofilms Microbiomes. 2017. 3:7. PMID: 28649408.

Bonn F, Maass S, Becher D. Sample preparation for mass spectrometry-based absolute protein quantification in antibiotic stress research. Methods Mol Biol. 2017. 1520:281-289. PMID: 27873259.

Hehemann JH, Truong LV, Unfried F, Welsch N, Kabisch J, Heiden SE, Junker S, Becher D, Thürmer A, Daniel R, Amann R, Schweder T. Aquatic adaptation of a laterally acquired pectin degradation pathway in marine gammaproteobacteria. Environ Microbiol. 2017. 19(6):2320-2333. PMID: 28276126.

Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S. Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis. ISME J. 2017. 11(2):463-477. PMID: 27801908.

Schmölders J, Manske C, Otto A, Hoffmann C, Steiner B, Welin A, Becher D, Hilbi H. Comparative proteomics of purified pathogen vacuoles correlates intracellular replication of Legionella pneumophila with the small GTPase Ras-related protein 1 (Rap1). Mol Cell Proteomics. 2017. 16(4):622-641. PMID: 28183814.

Giovannelli D, Sievert SM, Hügler M, Markert S, Becher D, Schweder T, Vetriani C. Insight into the evolution of microbial metabolism from the deep-branching bacterium, Thermovibrio ammonificans. Elife. 2017. 6:e18990. PMID: 28436819.

Hillion M, Bernhardt J, Busche T, Rossius M, Maaß S, Becher D, Rawat M, Wirtz M, Hell R, Rückert C, Kalinowski J, Antelmann H. Monitoring global protein thiol-oxidation and protein S-mycothiolation in Mycobacterium smegmatis under hypochlorite stress. Sci Rep. 2017. 7(1):1195. PMID: 28446771.

Handtke S, Albrecht D, Zühlke D, Otto A, Becher D, Schweder T, Riedel K, Hecker M, Voigt B. Bacillus pumilus KatX2 confers enhanced hydrogen peroxide resistance to a Bacillus subtilis PkatA::katX2 mutant strain. Microb Cell Fact. 2017. 16(1):72. PMID: 28446175.

Mekonnen SA, Palma Medina LM, Glasner C, Tsompanidou E, de Jong A, Grasso S, Schaffer M, Mäder U, Larsen AR, Gumpert H, Westh H, Völker U, Otto A, Becher D, van Dijl JM. Signatures of cytoplasmic proteins in the exoproteome distinguish community- and hospital-associated methicillin-resistant Staphylococcus aureus USA300 lineages. Virulence. 2017. 8(6):891-907. PMID: 28475476.

Eymann C, Lassek C, Wegner U, Bernhardt J, Fritsch OA, Fuchs S, Otto A, Albrecht D, Schiefelbein U, Cernava T, Aschenbrenner I, Berg G, Grube M, Riedel K. Symbiotic interplay of fungi, algae, and bacteria within the lung lichen Lobaria pulmonaria L. Hoffm. as assessed by state-of-the-art metaproteomics. J Proteome Res. 2017. 16(6):2160-2173. PMID: 28290203.


König S, Gros O, Heiden SE, Hinzke T, Thürmer A, Poehlein A, Meyer S, Vatin M, Mbéguié-A-Mbéguié D, Tocny J, Ponnudurai R, Daniel R, Becher D, Schweder T, Markert S. Nitrogen fixation in a chemoautotrophic lucinid symbiosis. Nat Microbiol. 2016. 2:16193. PMID: 27775698.

Stobernack T, Glasner C, Junker S, Gabarrini G, de Smit M, de Jong A, Otto A, Becher D, van Winkelhoff AJ, van Dijl JM. The extracellular proteome andc itrullinome of the oral pathogen Porphyromonas gingivalis. J Proteome Res. 2016. 15(12):4532-4543. PMID: 27712078.

Lappann M, Otto A, Brauer M, Becher D, Vogel U, Johswich K. Impact of moderate temperature changes on Neisseria meningitidis adhesive phenotypes and proteome. Infect Immun. 2016. 18;84(12):3484-3495. PMID: 27672084.

Smirnov A, Förstner KU, Holmqvist E, Otto A, Günster R, Becher D, Reinhardt R, Vogel J. Grad-seq guides the discovery of ProQ as a major small RNA-binding protein. Proc Natl Acad Sci U S A. 2016. 11;113(41):11591-11596. PMID: 27671629.

Otto A, Biran D, Sura T, Becher D, Ron EZ. Proteomics of septicemic Escherichia coli. Proteomics Clin Appl. 2016. 10(9-10):1020-1024. Review. PMID: 27604157.

Otto A, Maaß S, Lassek C, Becher D, Hecker M, Riedel K, Sievers S. The protein inventory of Clostridium difficile grown in complex and minimal medium. Proteomics Clin Appl. 2016. 10(9-10):1068-1072. PMID: 27511832.

Zühlke D, Dörries K, Bernhardt J, Maaß S, Muntel J, Liebscher V, Pané-Farré J, Riedel K, Lalk M, Völker U, Engelmann S, Becher D, Fuchs S, Hecker M. Costs of life – Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. Sci Rep. 2016.27;6:28172. PMID: 27344979.

López-Mondéjar R, Zühlke D, Větrovský T, Becher D, Riedel K, Baldrian P. Decoding the complete arsenal for cellulose and hemicellulose deconstruction in the highly efficient cellulose decomposer Paenibacillus O199. Biotechnol Biofuels. 2016. 9:104. PMID: 27186238.

Taha MK, Claus H, Lappann M, Veyrier FJ, Otto A, Becher D, Deghmane AE, Frosch M, Hellenbrand W, Hong E, Parent du Châtelet I, Prior K, Harmsen D, Vogel U. Evolutionary events associated with an outbreak of meningococcal disease in men who have sex with men. PLoS One. 2016. 11(5):e0154047. PMID: 27167067.

López-Mondéjar R, Zühlke D, Becher D, Riedel K, Baldrian P. Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems. Sci Rep. 2016. 29;6:25279. PMID: 27125755.

Herweg JA, Pons V, Becher D, Hecker M, Krohne G, Barbier J, Berger H, Rudel T, Mehlitz A. Proteomic analysis of the Simkania-containing vacuole: the central role of retrograde transport. Mol Microbiol. 2016. 99(1):151-71. PMID: 26374382.

Aurass P, Gerlach T, Becher D, Voigt B, Karste S, Bernhardt J, Riedel K, Hecker M, Flieger A. Life stage-specific proteomes of Legionella pneumophila reveal a highly differential abundance of virulence-associated Dot/Icm effectors. Mol Cell Proteomics. 2016. 15(1):177-200. PMID: 26545400.

Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters. J Proteomics. 2016. 131:140-8. PMID: 26597626.

Dietz S, Lassek C, Mack SL, Ritzmann M, Stadler J, Becher D, Hoelzle K, Riedel K, Hoelzle LE. Updating the proteome of the uncultivable hemotrophic Mycoplasma suis in experimentally infected pigs. Proteomics. 2016. 16(4):609-13. PMID: 26678042.

Maaß S, Becher D. Methods and applications of absolute protein quantification in microbial Systems. J Proteomics. 2016. 136:222-33. Review. PMID: 26825536.

Bonn F, Pané-Farré J, Schlüter R, Schaffer M, Fuchs S, Bernhardt J, Riedel K, Otto A, Völker U, Dijl JM, Hecker M, Mäder U, Becher D. Global analysis of the impact of linezolid onto virulence factor production in S. aureus USA300. Int J Med Microbiol. 2016. 306(3):131-40. PMID: 26996810.


Goelzer A, Muntel J, Chubukov V, Jules M, Prestel E, Nölker R, Mariadassou M, Aymerich S, Hecker M, Noirot P, Becher D, Fromion V. Quantitative prediction of genome-wide resource allocation in bacteria. Metab Eng. 2015. 32:232-43. PMID: 26498510.

Sayavedra L, Kleiner M, Ponnudurai R, Wetzel S, Pelletier E, Barbe V, Satoh N, Shoguchi E, Fink D, Breusing C, Reusch TB, Rosenstiel P, Schilhabel MB, Becher D, Schweder T, Markert S, Dubilier N, Petersen JM. Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels. Elife. 2015. 4. pii: e07966. PMID: 26371554.

Xing P, Hahnke RL, Unfried F, Markert S, Huang S, Barbeyron T, Harder J, Becher D, Schweder T, Glöckner FO, Amann RI, Teeling H. Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015. 9(6):1410-22. PMID: 25478683.

Singhal A, Arora G, Virmani R, Kundu P, Khanna T, Sajid A, Misra R, Joshi J, Yadav V, Samanta S, Saini N, Pandey AK, Visweswariah SS, Hentschker C, Becher D, Gerth U, Singh Y. Systematic analysis of mycobacterial acylation reveals first example of acylation-mediated regulation of enzyme activity of a bacterial phosphatase. J Biol Chem. 2015. 290(43):26218-34. PMID: 26350458.

Heazlewood JL, Schrimpf SP, Becher D, Riedel K, Tholey A, Bendixen E. Multi-Organism Proteomes (iMOP): Advancing our understanding of human biology. Proteomics. 2015. 15(17):2885-94. PMID: 26331910.

Theilacker C, Diederich AK, Otto A, Sava IG, Wobser D, Bao Y, Hese K, Broszat M, Henneke P, Becher D, Huebner J. Enterococcus faecalis glycolipids modulate lipoprotein-content of the bacterial cell membrane and host immune response. PLoS One. 2015. 10(7):e0132949. PMID: 26172831.

Neef J, Milder FJ, Koedijk DG, Klaassens M, Heezius EC, van Strijp JA, Otto A, Becher D, van Dijl JM, Buist G. Versatile vector suite for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis. Appl Microbiol Biotechnol. 2015. 99(21):9037-48. PMID: 26160391.

Moche M, Schlüter R, Bernhardt J, Plate K, Riedel K, Hecker M, Becher D. Time-resolved analysis of cytosolic and surface-associated proteins of Staphylococcus aureus HG001 under planktonic and biofilm conditions. J Proteome Res. 2015. 14(9):3804-22. PMID: 26152824.

Herweg JA, Hansmeier N, Otto A, Geffken AC, Subbarayal P, Prusty BK, Becher D, Hensel M, Schaible UE, Rudel T, Hilbi H. Purification and proteomics of pathogen-modified vacuoles and membranes. Front Cell Infect Microbiol. 2015. 5:48. Review. PMID: 26082896.

Kern B, Jain U, Utsch C, Otto A, Busch B, Jiménez-Soto L, Becher D, Haas R. Characterization of Helicobacter pylori VacA-containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes. Cell Microbiol. 2015. 17(12):1811-32. PMID: 26078003.

Stopnisek N, Zühlke D, Carlier A, Barberán A, Fierer N, Becher D, Riedel K, Eberl L and Weisskopf L. Molecular mechanisms underlying the close association between soil Burkholderia and fungi. ISME J. 2015. 10(1):253-64. PMID: 25989372.

Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. J Proteomics. 2015. 125:1-16. PMID: 25896738.

Prasse D, Thomsen J, De Santis R, Muntel J, Becher D, Schmitz RA. First description of small proteins encoded by spRNAs in Methanosarcina mazei strain Gö1. Biochimie. 2015. 117:138-48 PMID: 25890157.

Voigt B, Albrecht D, Sievers S, Becher D, Bongaerts J, Evers S, Schweder T, Maurer KH, Hecker M. High resolution proteome maps of Bacillus licheniformis cells growing in minimal medium. Proteomics. 2015. 15(15):2629-33. PMID: 25867794.

Brack C, Mikolasch A, Schlueter R, Otto A, Becher D, Wegner U, Albrecht D, Riedel K, Schauer F. Antibacterial metabolites and bacteriolytic enzymes produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. Mar Biotechnol (NY). 2015. 17(3):290-304. PMID: 25678259.

Lassek C, Burghartz M, Chaves-Moreno D, Otto A, Hentschker C, Fuchs S, Bernhardt J, Jauregui R, Neubauer R, Becher D, Pieper DH, Jahn M, Jahn D, Riedel K. A Metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (CAUTIs). Mol Cell Proteomics. 2015. 14(4):989-1008. PMID: 25673765.

Salzberg LI, Botella E, Hokamp K, Antelmann H, Maaß S, Becher D, Noone D, Devine KM. Genome-wide analysis of phosphorylated PhoP binding to chromosomal DNA reveals several novel features of the PhoPR-mediated phosphate limitation response in Bacillus subtilis. J Bacteriol. 2015. 197(8):1492-506. PMID: 25666134.

AbouElfetouh A, Kuhn ML, Hu LI, Scholle MD, Sorensen DJ, Sahu AK, Becher D, Antelmann H, Mrksich M, Anderson WF, Gibson BW, Schilling B, Wolfe AJ. The E. coli sirtuin CobB shows no preference for enzymatic and nonenzymatic lysine acetylation substrate sites. Microbiologyopen. 2015. 4(1):66-83. PMID: 25417765.


Handtke S, Volland S, Methling K, Albrecht D, Becher D, Nehls J, Bongaerts J, Maurer KH, Lalk M, Liesegang H, Voigt B, Daniel R, Hecker M. Cell physiology of the biotechnological relevant bacterium Bacillus pumilus-An omics-based approach. J Biotechnol. 2014. 192 Pt A:204-14. PMID: 25281541.

Kohlmann Y, Pohlmann A, Schwartz E, Zühlke D, Otto A, Albrecht D, Grimmler C, Ehrenreich A, Voigt B, Becher D, Hecker M, Friedrich B, Cramm R. Coping with Anoxia: A comprehensive proteomic and transcriptomic survey of denitrification. J Proteome Res. 2014. 13(10):4325-38. PMID: 25198380.

Maaβ S, Wachlin G, Bernhardt J, Eymann C, Fromion V, Riedel K, Becher D, Hecker M. Highly precise quantification of protein molecules per cell during stress and starvation responses in Bacillus subtilis. Mol Cell Proteomics. 2014. 13(9):2260-76. PMID: 24878497.

Huja S, Oren Y, Biran D, Meyer S, Dobrindt U, Bernhard J, Becher D, Hecker M, Sorek R, Ron EZ. Fur is the master regulator of the extraintestinal pathogenic Escherichia coli response to serum. MBio. 2014. 5(4). pii: e01460-14. PMID: 25118243.

Bonn F, Bartel J, Büttner K, Hecker M, Otto A, Becher D. Picking vanished proteins from the void: how to collect and ship/share extremely dilute proteins in a reproducible and highly efficient manner. Anal Chem. 2014. 86(15):7421-7. PMID: 24987932.

Grube M, Cernava T, Soh J, Fuchs S, Aschenbrenner I, Lassek C, Wegner U, Becher D, Riedel K, Sensen CW, Berg G. Exploring functional contexts of symbiotic sustain within lichen-associated bacteria by comparative omics. ISME J. 2014. doi: 10.1038/ismej.2014.138. PMID: 25072413.

Gaballa A, Chi BK, Roberts AA, Becher D, Hamilton CJ, Antelmann H, Helmann JD. Redox regulation in Bacillus subtilis: The bacilliredoxins BrxA (YphP) and BrxB (YqiW) function in de-bacillithiolation of S-bacillithiolated OhrR and MetE. Antioxid Redox Signal. 2014. 21(3):357-67. PMID: 24313874.

Hoffmann C, Finsel I, Otto A, Pfaffinger G, Rothmeier E, Hecker M, Becher D, Hilbi H. Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages. Cell Microbiol. 2014. 16(7):1034-52. PMID: 24373249.

Kabisch A, Otto A, König S, Becher D, Albrecht D, Schüler M, Teeling H, Amann RI, Schweder T. Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes ‘Gramella forsetii‘ KT0803. ISME J. 2014. 8(7):1492-502. PubMed PMID: 24522261.

Petasch J, Disch EM, Markert S, Becher D, Schweder T, Hüttel B, Reinhardt R, Harder J. The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen. BMC Microbiol. 2014. 14:164. PMID: 24952578.

Wenzel M, Chiriac AI, Otto A, Zweytick D, May C, Schumacher C, Gust R, Albada HB, Penkova M, Krämer U, Erdmann R, Metzler-Nolte N, Straus SK, Bremer E, Becher D, Brötz-Oesterhelt H, Sahl HG, Bandow JE. Small cationic antimicrobial peptides delocalize peripheral membrane proteins. Proc Natl Acad Sci U S A. 2014. 111(14):E1409-18. PMID: 24706874.

Muntel J, Fromion V, Goelzer A, Maaβ S, Mäder U, Büttner K, Hecker M, Becher D. Comprehensive absolute quantification of the cytosolic proteome of Bacillus subtilis by data independent, parallel fragmentation in liquid chromatography/mass spectrometry (LC/MS(E)). Mol Cell Proteomics. 2014. 13(4):1008-19. PMID: 24696501.

Bäsell K, Otto A, Junker S, Zühlke D, Rappen GM, Schmidt S, Hentschker C, Macek B, Ohlsen K, Hecker M, Becher D. The phosphoproteome and its physiological dynamics in Staphylococcus aureus. Int J Med Microbiol. 2014. 304(2):121-32. PMID: 24457182.

Otto A, van Dijl JM, Hecker M, Becher D. The Staphylococcus aureus proteome. Int J Med Microbiol. 2014. 304(2):110-20. Review. PMID: 24439828.

Otto A, Becher D, Schmidt F. Quantitative proteomics in the field of microbiology. Proteomics. 2014. 14(4-5):547-65. Review. PMID: 24376008.

Pribyl T, Moche M, Dreisbach A, Bijlsma JJ, Saleh M, Abdullah MR, Hecker M, van Dijl JM, Becher D, Hammerschmidt S. Influence of impaired lipoprotein biogenesis on surface and exoproteome of Streptococcus pneumoniae. J Proteome Res. 2014. 13(2):650-67. PMID: 24387739.

Chi BK, Busche T, Van Laer K, Bäsell K, Becher D, Clermont L, Seibold GM, Persicke M, Kalinowski J, Messens J, Antelmann H. Protein S-mycothiolation functions as redox-switch and thiol protection mechanism in Corynebacterium glutamicum under hypochlorite stress. Antioxid Redox Signal. 2014. 20(4):589-605. PMID: 23886307.

Noone D, Salzberg LI, Botella E, Bäsell K, Becher D, Antelmann H, Devine KM. A highly unstable transcript makes CwlO D,L-endopeptidase expression responsive to growth conditions in Bacillus subtilis. J Bacteriol. 2014. 196(2):237-47. PMID: 24163346.

Miranda HV, Antelmann H, Hepowit N, Chavarria NE, Krause DJ, Pritz JR, Bäsell K, Becher D, Humbard MA, Brocchieri L, Maupin-Furlow JA. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism. Mol Cell Proteomics. 2014. 13(1):220-39. PMID: 24097257.


Hessling B, Bonn F, Otto A, Herbst FA, Rappen GM, Bernhardt J, Hecker M, Becher D. Global proteome analysis of vancomycin stress in Staphylococcus aureus. Int J Med Microbiol. 2013. 303(8):624-34. PMID: 24161710.

Greiner R, Pálinkás Z, Bäsell K, Becher D, Antelmann H, Nagy P, Dick TP. Polysulfides link H2S to protein thiol oxidation. Antioxid Redox Signal. 2013. 19(15):1749-65.
PMID: 23646934.

Lappann M, Otto A, Becher D, Vogel U. Comparative proteome analysis of spontaneous outer membrane vesicles and purified outer membranes of Neisseria meningitidis. J Bacteriol. 2013. 195(19):4425-35. PMID: 23893116.

Hessling B, Büttner K, Hecker M, Becher D. Global relative quantification with liquid chromatography-matrix-assisted laser desorption ionization time-of-flight (LC-MALDI-TOF)–cross-validation with LTQ-Orbitrap proves reliability and reveals complementary ionization preferences. Mol Cell Proteomics. 2013. 12(10):2911-20.
PMID: 23788530.

Becher D, Bernhardt J, Fuchs S, Riedel K. Metaproteomics to unravel major microbial players in leaf litter and soil environments: challenges and perspectives. Proteomics. 2013. 13(18-19):2895-909. Review. PMID: 23894095.

Krishnappa L, Dreisbach A, Otto A, Goosens VJ, Cranenburgh RM, Harwood CR, Becher D, van Dijl JM. Extracytoplasmic proteases determining the cleavage and release of secreted proteins, lipoproteins, and membrane proteins in Bacillus subtilis. J Proteome Res. 2013. 12(9):4101-10. PMID: 23937099.

Hu LI, Chi BK, Kuhn ML, Filippova EV, Walker-Peddakotla AJ, Bäsell K, Becher D, Anderson WF, Antelmann H, Wolfe AJ. Acetylation of the response regulator RcsB controls transcription from a small RNA promoter. J Bacteriol. 2013. 195(18):4174-86. PMID: 23852870.

Musa YR, Bäsell K, Schatschneider S, Vorhölter FJ, Becher D, Niehaus K. Dynamic protein phosphorylation during the growth of Xanthomonas campestris pv. campestris B100 revealed by a gel-based proteomics approach. J Biotechnol. 2013. 167(2):111-22. PMID: 23792782.

Chi BK, Roberts AA, Huyen TT, Bäsell K, Becher D, Albrecht D, Hamilton CJ, Antelmann H. S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria. Antioxid Redox Signal. 2013. 18(11):1273-95. PMID: 22938038.

Tsompanidou E, Denham EL, Becher D, de Jong A, Buist G, van Oosten M, Manson WL, Back JW, van Dijl JM, Dreisbach A. Distinct roles of phenol-soluble modulins in spreading of Staphylococcus aureus on wet surfaces. Appl Environ Microbiol. 2013. 79(3):886-95. PMID: 23183971.

Goosens VJ, Otto A, Glasner C, Monteferrante CC, van der Ploeg R, Hecker M, Becher D, van Dijl JM. Novel twin-arginine translocation pathway-dependent phenotypes of Bacillus subtilis unveiled by quantitative proteomics. J Proteome Res. 2013. 12(2):796-807. PMID: 23256564.

Schroeter R, Hoffmann T, Voigt B, Meyer H, Bleisteiner M, Muntel J, Jürgen B, Albrecht D, Becher D, Lalk M, Evers S, Bongaerts J, Maurer KH, Putzer H, Hecker M, Schweder T, Bremer E. Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges. PLoS One. 2013. 8(11):e80956. PMID: 24348917.

Celik H, Blouzard JC, Voigt B, Becher D, Trotter V, Fierobe HP, Tardif C, Pagès S, de Philip P. A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. PLoS One. 2013. 8(2):e56063. PMID: 23418511.


Sibbald MJ, Yang XM, Tsompanidou E, Qu D, Hecker M, Becher D, Buist G, van Dijl JM. Partially overlapping substrate specificities of staphylococcal group A sortases. Proteomics. 2012. 12(19-20):3049-62. PMID: 22930668.

Lima BP, Thanh Huyen TT, Bäsell K, Becher D, Antelmann H, Wolfe AJ. Inhibition of acetyl phosphate-dependent transcription by an acetylatable lysine on RNA polymerase. J Biol Chem. 2012. 287(38):32147-60. PMID: 22829598.

Michalik S, Bernhardt J, Otto A, Moche M, Becher D, Meyer H, Lalk M, Schurmann C, Schlüter R, Kock H, Gerth U, Hecker M. Life and death of proteins: a case study of glucose-starved Staphylococcus aureus. Mol Cell Proteomics. 2012. 11(9):558-70. PMID: 22556279.

Otto A, Bernhardt J, Hecker M, Becher D. Global relative and absolute quantitation in microbial proteomics. Curr Opin Microbiol. 2012. 15(3):364-72. Review.
PMID: 22445110.

Voigt B, Hieu CX, Hempel K, Becher D, Schlüter R, Teeling H, Glöckner FO, Amann R, Hecker M, Schweder T. Cell surface proteome of the marine planctomycete Rhodopirellula baltica. Proteomics. 2012. 12(11):1781-91. PMID: 22623273.

Kleiner M, Wentrup C, Lott C, Teeling H, Wetzel S, Young J, Chang YJ, Shah M, VerBerkmoes NC, Zarzycki J, Fuchs G, Markert S, Hempel K, Voigt B, Becher D, Liebeke M, Lalk M, Albrecht D, Hecker M, Schweder T, Dubilier N. Metaproteomics of a gutless marine worm and its symbiotic microbial community reveal unusual pathways for carbon and energy use. Proc Natl Acad Sci U S A. 2012. 109(19):E1173-82. PMID: 22517752.

Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U. Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis. Proc Natl Acad Sci U S A. 2012. 109(19):7451-6. PMID: 22517742.

Teeling H, Fuchs BM, Becher D, Klockow C, Gardebrecht A, Bennke CM, Kassabgy M, Huang S, Mann AJ, Waldmann J, Weber M, Klindworth A, Otto A, Lange J, Bernhardt J, Reinsch C, Hecker M, Peplies J, Bockelmann FD, Callies U, Gerdts G, Wichels A, Wiltshire KH, Glöckner FO, Schweder T, Amann R. Substrate-controlled succession of marine bacterioplankton populations induced by a phytoplankton bloom. Science. 2012. 336(6081):608-11. PMID: 22556258.

Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H. Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR. Nucleic Acids Res. 2012. 40(9):4178-92. PMID: 22238377.

Muntel J, Hecker M, Becher D. An exclusion list based label-free proteome quantification approach using an LTQ Orbitrap. Rapid Commun Mass Spectrom. 2012. 26(6):701-9. PMID: 22328225.

Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P. Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science. 2012. 335(6072):1103-6. PMID: 22383849.

Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, Hessling B, Kleijn RJ, Le Chat L, Lecointe F, Mäder U, Nicolas P, Piersma S, Rügheimer F, Becher D, Bessieres P, Bidnenko E, Denham EL, Dervyn E, Devine KM, Doherty G, Drulhe S, Felicori L, Fogg MJ, Goelzer A, Hansen A, Harwood CR, Hecker M, Hubner S, Hultschig C, Jarmer H, Klipp E, Leduc A, Lewis P, Molina F, Noirot P, Peres S, Pigeonneau N, Pohl S, Rasmussen S, Rinn B, Schaffer M, Schnidder J, Schwikowski B, Van Dijl JM, Veiga P, Walsh S, Wilkinson AJ, Stelling J, Aymerich S, Sauer U. Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism. Science. 2012. 335(6072):1099-103. PMID: 22383848.

Heinz E, Williams TA, Nakjang S, Noël CJ, Swan DC, Goldberg AV, Harris SR, Weinmaier T, Markert S, Becher D, Bernhardt J, Dagan T, Hacker C, Lucocq JM, Schweder T, Rattei T, Hall N, Hirt RP, Embley TM. The genome of the obligate intracellular parasite Trachipleistophora hominis: new insights into microsporidian genome dynamics and reductive evolution. PLoS Pathog. 2012. 8(10):e1002979. PMID: 23133373.


Chi BK, Gronau K, Mäder U, Hessling B, Becher D, Antelmann H. S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics. Mol Cell Proteomics. 2011. 10(11):M111.009506. PMID: 21749987.

Miller M, Dreisbach A, Otto A, Becher D, Bernhardt J, Hecker M, Peppelenbosch MP, van Dijl JM. Mapping of interactions between human macrophages and Staphylococcus aureus reveals an involvement of MAP kinase signaling in the host defense. J Proteome Res. 2011. 10(9):4018-32. PMID: 21736355.

Lima BP, Antelmann H, Gronau K, Chi BK, Becher D, Brinsmade SR, Wolfe AJ. Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter. Mol Microbiol. 2011. 81(5):1190-204. PMID: 21696463.

Schmidl SR, Otto A, Lluch-Senar M, Piñol J, Busse J, Becher D, Stülke J. A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression. PLoS Pathog. 2011. 7(9):e1002263. PMID: 21966272.

Elsholz AK, Hempel K, Michalik S, Gronau K, Becher D, Hecker M, Gerth U. Activity control of the ClpC adaptor McsB in Bacillus subtilis. J Bacteriol. 2011. 193(15):3887-93. PMID: 21622759.

Markert S, Gardebrecht A, Felbeck H, Sievert SM, Klose J, Becher D, Albrecht D, Thürmer A, Daniel R, Kleiner M, Hecker M, Schweder T. Status quo in physiological proteomics of the uncultured Riftia pachyptila endosymbiont. Proteomics. 2011. 11(15):3106-17. PMID: 21710568.

Delumeau O, Lecointe F, Muntel J, Guillot A, Guédon E, Monnet V, Hecker M, Becher D, Polard P, Noirot P. The dynamic protein partnership of RNA polymerase in Bacillus subtilis. Proteomics. 2011. 11(15):2992-3001. PMID: 21710567.

Becher D, Büttner K, Moche M, Hessling B, Hecker M. From the genome sequence to the protein inventory of Bacillus subtilis. Proteomics. 2011. 11(15):2971-80. Review. PMID: 21710564.

Dreisbach A, van der Kooi-Pol MM, Otto A, Gronau K, Bonarius HP, Westra H, Groen H, Becher D, Hecker M, van Dijl JM. Surface shaving as a versatile tool to profile global interactions between human serum proteins and the Staphylococcus aureus cell surface. Proteomics. 2011. 11(14):2921-30. PMID: 21674804.

Albaum SP, Hahne H, Otto A, Haußmann U, Becher D, Poetsch A, Goesmann A, Nattkemper TW. A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study. Proteome Sci. 2011. 9:30. PMID: 21663690.

Kohlmann Y, Pohlmann A, Otto A, Becher D, Cramm R, Lütte S, Schwartz E, Hecker M, Friedrich B. Analyses of soluble and membrane proteomes of Ralstonia eutropha H16 reveal major changes in the protein complement in adaptation to lithoautotrophy. J Proteome Res. 2011. 10(6):2767-76. PMID: 21561103.

Eymann C, Schulz S, Gronau K, Becher D, Hecker M, Price CW. In vivo phosphorylation patterns of key stressosome proteins define a second feedback loop that limits activation of Bacillus subtilis σB. Mol Microbiol. 2011. 80(3):798-810. PMID: 21362065.

Tefon BE, Maass S, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. A comprehensive analysis of Bordetella pertussis surface proteome and identification of new immunogenic proteins. Vaccine. 2011. 29(19):3583-95. PMID: 21397717.

Maass S, Sievers S, Zühlke D, Kuzinski J, Sappa PK, Muntel J, Hessling B, Bernhardt J, Sietmann R, Völker U, Hecker M, Becher D. Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011. 83(7):2677-84.                                PMID: 21395229.

Hempel K, Herbst FA, Moche M, Hecker M, Becher D. Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions. J Proteome Res. 2011. 10(4):1657-66. PMID: 21323324.

Yıldırım V, Ozcan S, Becher D, Büttner K, Hecker M, Ozcengiz G. Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure. Proteome Sci. 2011. 9:12. PMID: 21388532.

Elsholz AK, Hempel K, Pöther DC, Becher D, Hecker M, Gerth U. CtsR inactivation during thiol-specific stress in low GC, Gram+ bacteria. Mol Microbiol. 2011. 79(3):772-85. PMID: 21208299.

Saller MJ, Otto A, Berrelkamp-Lahpor GA, Becher D, Hecker M, Driessen AJ. Bacillus subtilis YqjG is required for genetic competence development. Proteomics. 2011. 11(2):270-82. PMID: 21204254.


Price CE, Otto A, Fusetti F, Becher D, Hecker M, Driessen AJ. Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. Proteomics. 2010. 10(18):3235-47. PMID: 20706981.

Dreisbach A, Hempel K, Buist G, Hecker M, Becher D, van Dijl JM. Profiling the surfacome of Staphylococcus aureus. Proteomics. 2010. 10(17):3082-96.                              PMID: 20662103.

Chi BK, Albrecht D, Gronau K, Becher D, Hecker M, Antelmann H. The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis. Proteomics. 2010. 10(17):3155-64. PMID: 20652907.

Hyyryläinen HL, Marciniak BC, Dahncke K, Pietiäinen M, Courtin P, Vitikainen M, Seppala R, Otto A, Becher D, Chapot-Chartier MP, Kuipers OP, Kontinen VP. Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis. Mol Microbiol. 2010. 77(1):108-27. PMID: 20487272.

Möbius K, Arias-Cartin R, Breckau D, Hännig AL, Riedmann K, Biedendieck R, Schröder S, Becher D, Magalon A, Moser J, Jahn M, Jahn D. Heme biosynthesis is coupled to electron transport chains for energy generation. Proc Natl Acad Sci U S A. 2010. 107(23):10436-41. PMID: 20484676.

Schmidl SR, Gronau K, Pietack N, Hecker M, Becher D, Stülke J. The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases. Mol Cell Proteomics. 2010. 9(6):1228-42. PMID: 20097688.

Sievers S, Ernst CM, Geiger T, Hecker M, Wolz C, Becher D, Peschel A. Changing the phospholipid composition of Staphylococcus aureus causes distinct changes in membrane proteome and membrane-sensory regulators. Proteomics. 2010. 10(8):1685-93. PMID: 20162562.

Hempel K, Pané-Farré J, Otto A, Sievers S, Hecker M, Becher D. Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach. J Proteome Res. 2010. 9(3):1579-90. PMID: 20108986.

Miller M, Donat S, Rakette S, Stehle T, Kouwen TR, Diks SH, Dreisbach A, Reilman E, Gronau K, Becher D, Peppelenbosch MP, van Dijl JM, Ohlsen K. Staphylococcal PknB as the first prokaryotic representative of the proline-directed kinases. PLoS One. 2010. 5(2):e9057. PMID: 20140229.

Hecker M, Becher D, Fuchs S, Engelmann S. A proteomic view of cell physiology and virulence of Staphylococcus aureus. Int J Med Microbiol. 2010. 300(2-3):76-87 Review. PMID: 20005169.

Hahne H, Mäder U, Otto A, Bonn F, Steil L, Bremer E, Hecker M, Becher D. A comprehensive proteomics and transcriptomics analysis of Bacillus subtilis salt stress adaptation. J Bacteriol. 2010. 192(3):870-82. PMID: 19948795.

Schmidl SR, Gronau K, Hames C, Busse J, Becher D, Hecker M, Stülke J. The stability of cytadherence proteins in Mycoplasma pneumoniae requires activity of the protein kinase PrkC. Infect Immun. 2010. 78(1):184-92. PMID: 19858294.

Pietack N, Becher D, Schmidl SR, Saier MH, Hecker M, Commichau FM, Stülke J. In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism. J Mol Microbiol Biotechnol. 2010. 18(3):129-40. PMID: 20389117.

Otto A, Bernhardt J, Meyer H, Schaffer M, Herbst FA, Siebourg J, Mäder U, Lalk M, Hecker M, Becher D. Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis. Nat Commun. 2010. 1:137. PMID: 21266987.


Becher D, Hempel K, Sievers S, Zühlke D, Pané-Farré J, Otto A, Fuchs S, Albrecht D, Bernhardt J, Engelmann S, Völker U, van Dijl JM, Hecker M. A proteomic view of an important human pathogen–towards the quantification of the entire Staphylococcus aureus proteome. PLoS One. 2009. 4(12):e8176. PMID: 19997597.

Pöther DC, Liebeke M, Hochgräfe F, Antelmann H, Becher D, Lalk M, Lindequist U, Borovok I, Cohen G, Aharonowitz Y, Hecker M. Diamide triggers mainly S Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus. J Bacteriol. 2009. 191(24):7520-30. PMID: 19837798.

Hempel K, Rosen R, Becher D, Büttner K, Hecker M, Ron EZ. Analysis of ultra acidic proteins by the use of anodic acidic gels. Anal Biochem. 2009. 385(2):208-14.
PMID: 19084495.

Altindiş E, Tefon BE, Yildirim V, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins. Vaccine. 2009. 27(4):542-8. PMID: 19028538.


Hahne H, Wolff S, Hecker M, Becher D. From complementarity to comprehensiveness–targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics. 2008. 8(19):4123-36. PMID: 18763711.

Liebeke M, Pöther DC, van Duy N, Albrecht D, Becher D, Hochgräfe F, Lalk M, Hecker M, Antelmann H. Depletion of thiol-containing proteins in response to quinones in Bacillus subtilis. Mol Microbiol. 2008. 69(6):1513-29. PMID: 18673455.

Wolff S, Hahne H, Hecker M, Becher D. Complementary analysis of the vegetative membrane proteome of the human pathogen Staphylococcus aureus. Mol Cell Proteomics. 2008. 7(8):1460-8. PMID: 18460691.

Dreisbach A, Otto A, Becher D, Hammer E, Teumer A, Gouw JW, Hecker M, Völker U. Monitoring of changes in the membrane proteome during stationary phase adaptation of Bacillus subtilis using in vivo labeling techniques. Proteomics. 2008. 8(10):2062-76. PMID: 18491319.

Zweers JC, Barák I, Becher D, Driessen AJ, Hecker M, Kontinen VP, Saller MJ, Vavrová L, van Dijl JM. Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes. Microb Cell Fact. 2008. 7:10. PMID: 18394159.

Hieu CX, Voigt B, Albrecht D, Becher D, Lombardot T, Glöckner FO, Amann R, Hecker M, Schweder T. Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltica SH1T. Proteomics. 2008. 8(8):1608-23. PMID: 18340632.

Leelakriangsak M, Huyen NT, Töwe S, van Duy N, Becher D, Hecker M, Antelmann H, Zuber P. Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis. Mol Microbiol. 2008. 67(5):1108-24. PMID: 18208493.


Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M. Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis. Proteomics. 2007. 7(19):3509-26. PMID: 17726680.

Hochgräfe F, Mostertz J, Pöther DC, Becher D, Helmann JD, Hecker M. S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress. J Biol Chem. 2007. 282(36):25981-5. PMID: 17611193.

Wolff S, Antelmann H, Albrecht D, Becher D, Bernhardt J, Bron S, Büttner K, van Dijl JM, Eymann C, Otto A, Tam le T, Hecker M. Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches. J Chromatogr B Analyt Technol Biomed Life Sci. 2007. 849(1-2):129-40. Review. PMID: 17055787.

Ozcan S, Yildirim V, Kaya L, Albrecht D, Becher D, Hecker M, Ozcengiz G. Phanerochaete chrysosporium soluble proteome as a prelude for the analysis of heavy metal stress response. Proteomics. 2007. 7(8):1249-60. PMID: 17366474.

Markert S, Arndt C, Felbeck H, Becher D, Sievert SM, Hügler M, Albrecht D, Robidart J, Bench S, Feldman RA, Hecker M, Schweder T. Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila. Science. 2007. 315(5809):247-50. PMID: 17218528.

Borriss M, Lombardot T, Glöckner FO, Becher D, Albrecht D, Schweder T. Genome and proteome characterization of the psychrophilic Flavobacterium bacteriophage 11b. Extremophiles. 2007. 11(1):95-104. PMID: 16932843.

2006 and older

Linsen L, Löcherbach J, Berth M, Becher D, Bernhardt J. Visual analysis of gel-free proteome data. IEEE Trans Vis Comput Graph. 2006. 12(4):497-508.                              PMID: 16805259.

Wolff S, Otto A, Albrecht D, Zeng JS, Büttner K, Glückmann M, Hecker M, Becher D. Gel-free and gel-based proteomics in Bacillus subtilis: a comparative study. Mol Cell Proteomics. 2006. 5(7):1183-92. PMID: 16552027.

Mattow J, Siejak F, Hagens K, Becher D, Albrecht D, Krah A, Schmidt F, Jungblut PR, Kaufmann SH, Schaible UE. Proteins unique to intraphagosomally grown Mycobacterium tuberculosis. Proteomics. 2006. 6(8):2485-94. PMID: 16548060.

Kohler C, Wolff S, Albrecht D, Fuchs S, Becher D, Büttner K, Engelmann S, Hecker M. Proteome analyses of Staphylococcus aureus in growing and non-growing cells: a physiological approach. Int J Med Microbiol. 2005. 295(8):547-65. PMID: 16325551.

Wesche J, Hammer E, Becher D, Burchhardt G, Schauer F. The bphC gene-encoded 2,3-dihydroxybiphenyl-1,2-dioxygenase is involved in complete degradation of dibenzofuran by the biphenyl-degrading bacterium Ralstonia sp. SBUG 290. J Appl Microbiol. 2005. 98(3):635-45. PMID: 15715866.

Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Probing the active site of homoserine trans-succinylase. FEBS Lett. 2004. 577(3):386-92. PMID: 15556615.

Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Highly phosphorylated bacterial proteins. Proteomics. 2004. 4(10):3068-77. PMID: 15378765.

Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M. A comprehensive proteome map of growing Bacillus subtilis cells. Proteomics. 2004. 4(10):2849-76. PMID: 15378759.

Ziebandt AK, Becher D, Ohlsen K, Hacker J, Hecker M, Engelmann S. The influence of agr and sigmaB in growth phase dependent regulation of virulence factors in Staphylococcus aureus. Proteomics. 2004. 4(10):3034-47. PMID: 15378746.

Voigt B, Schweder T, Becher D, Ehrenreich A, Gottschalk G, Feesche J, Maurer KH, Hecker M. A proteomic view of cell physiology of Bacillus licheniformis. Proteomics. 2004. 4(5):1465-90. PMID: 15188415.

Weber H, Engelmann S, Becher D, Hecker M. Oxidative stress triggers thiol oxidation in the glyceraldehyde-3-phosphate dehydrogenase of Staphylococcus aureus. Mol Microbiol. 2004. 52(1):133-40. PMID: 15049816.

Rosen R, Sacher A, Shechter N, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Two-dimensional reference map of Agrobacterium tumefaciens proteins. Proteomics. 2004. 4(4):1061-73. PMID: 15048987.

Brandt R, Nawka M, Kellermann J, Salazar R, Becher D, Krantz S. Nucleophosmin is a component of the fructoselysine-specific receptor in cell membranes of Mono Mac 6 and U937 monocyte-like cells. Biochim Biophys Acta. 2004. 1670(2):132-6. PMID: 14738996.

Krah A, Schmidt F, Becher D, Schmid M, Albrecht D, Rack A, Büttner K, Jungblut PR. Analysis of automatically generated peptide mass fingerprints of cellular proteins and antigens from Helicobacter pylori 26695 separated by two-dimensional electrophoresis. Mol Cell Proteomics. 2003. 2(12):1271-83. PMID: 14519719.

Rosen R, Matthysse AG, Becher D, Biran D, Yura T, Hecker M, Ron EZ. Proteome analysis of plant-induced proteins of Agrobacterium tumefaciens. FEMS Microbiol Ecol. 2003. 44(3):355-60. PMID: 19719616.

Bandow JE, Becher D, Büttner K, Hochgräfe F, Freiberg C, Brötz H, Hecker M. The role of peptide deformylase in protein biosynthesis: a proteomic study. Proteomics. 2003. 3(3):299-306. PMID: 12627383.

Stope MB, Becher D, Hammer E, Schauer F. Cometabolic ring fission of dibenzofuran by Gram-negative and Gram-positive biphenyl-utilizing bacteria. Appl Microbiol Biotechnol. 2002. 59(1):62-7. PMID: 12073133.

Rosen R, Büttner K, Becher D, Nakahigashi K, Yura T, Hecker M, Ron EZ. Heat shock proteome of Agrobacterium tumefaciens: evidence for new control systems. J Bacteriol. 2002. 184(6):1772-8. PMID: 11872730.

Rosen R, Biran D, Gur E, Becher D, Hecker M, Ron EZ. Protein aggregation in Escherichia coli: role of proteases. FEMS Microbiol Lett. 2002. 207(1):9-12.                             PMID: 11886743.

Becher D, Specht M, Hammer E, Francke W, Schauer F. Cometabolic degradation of dibenzofuran by biphenyl-cultivated Ralstonia sp. strain SBUG 290. Appl Environ Microbiol. 2000. 66(10):4528-31. PMID: 11010910.

Scheller U, Zimmer T, Becher D, Schauer F, Schunck WH. Oxygenation cascade in conversion of n-alkanes to alpha,omega-dioic acids catalyzed by cytochrome P450 52A3. J Biol Chem. 1998. 273(49):32528-34. PMID: 9829987.

Hundt K, Wagner M, Becher D, Hammer E, Schauer F. Effect of selected environmental factors on degradation and mineralization of biaryl compounds by the bacterium Ralstonia pickettii in soil and compost. Chemosphere. 1998. 36(10):2321-35. PMID: 9566302.